This study examined a survey of the challenges facing Biology learners in Distance Learning Institute, University of Lagos. The study was conducted using qualitative and quantitative approaches. The population of study comprised all Biology Education learners while a sample size of one hundred learners was obtained using stratified random sampling technique. Data was collected using structured questionnaire. Frequency table, bar charts and percentages were the main descriptive statistics used to analyze and present the results. Findings showed that a large percentage of learners (85%) do not have the technological know-how for the electronic mode of the program, there are lapses of effective interaction between the learners and facilitators. The study recommended that the learners’ support unit should be empowered to train and retrain learners who are digital immigrant adults on the e-learning mode. The system need to encourage effective interaction and feedback mechanisms between learners and facilitators. // Paper ID 95